A simple and sensitive high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of the levosulpiride and pantoprazole in their combined capsule dosage form. The chromatograms were developed using a mobile phase of ethyl acetate : toluene: methanol : Ammonia (5.5 : 3.0 : 1.5 : 0.2 v/v/v) on pre-coated plate of silica gel GF aluminium TLC plate and quantified by densitometric absorbance mode at 291 nm. This system was found to give compact spots for levosulpiride (Rf value of 0.24 ± 0.003) and pantoprazole (Rf value of 0.54 ± 0.008). The method was validated in terms of linearity, precision, accuracy, limit of detection, limit of quantification and specificity. The calibration curves were found to be linear between 100-500 ng/spot and 50-250 ng/spot for levosulpiride and pantoprazole, respectively. The proposed method was found to be reliable, reproducible and specific for the simultaneous estimation of levosulpiride and pantoprazole in their combined capsule dosage form.
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